Deepnest_37

Even better strat for 3 damage tanks. Saves another 0.25ish over the previous fastest strat.

Saves 0.5 over not damage tanking at the end, since you are probably taking tram bench for the heal before colos, health shouldn't matter

Edit:
Update for future readers, i get the 3 damage tank version (original post) to be roughly 0.04 faster in my testing, but more importantly it's easy to accidentally dash into the back of the last garpede with stratus' version, whereas original post strat is basically unmissable.

However, if you're bold you can skip the tram bench in the next room and just carry 4hp all the way through KE to colos. For any% SS definitely use the stratus version, for zote use the 3 damage tank version if you are going to bench, or stratus version if you are going to skip bench in tram and do 4hp KE

Sharpshadow movement through deepnest_37 on the way to tram. low 8.5 with good execution, ikc strat is low 8.7 - .2s timesave over it. Only minor points are to not bonk after the walk section, and to be sure to dash high enough that you don't corner correct (otherwise the sharp shadow recharges before second dash after damagetank).

2 dashes + neutral dash at bottom and inv knock back cancel to get only 1 hit in this room
https://youtu.be/ziEanlmd7OE